Significance of the Corynebacterium Glutamicum Msccg Mechanosensitive Channel in Fine-tuning of Compatible Solute Accumulation
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چکیده
Accessible online at: www.karger.com/cpb SIGNIFICANCE OF THE CORYNEBACTERIUM GLUTAMICUM MSCCG MECHANOSENSITIVE CHANNEL IN FINE-TUNING OF COMPATIBLE SOLUTE ACCUMULATION Kirsten Börngen1, Andrew R. Battle2,3, Nina Möker1, Susanne Morbach1, Kay Marin1, Boris Martinac3 and Reinhard Krämer1 1University of Cologne, Köln, Germany, 2Griffith University, Parklands, Queensland, Australia, 3Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia
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A gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion.
The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl12...
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